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Showing posts from August, 2018

HAY'S TEST FOR BILE SALTS

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HAY'S TEST FOR BILE SALTS Hay’s test for bile salts – Its principle, reagent and procedure Hay’s test for bile salts: Hay’s test for bile salts is a specific test used for the qualitative detection of bile salts in urine. Bile salt appears in the urine of patients suffering from jaundice. It consists of a watery mixture of organic and inorganic compounds. Bile acids are cholic acid and chenodeoxycholic acid which form conjugation with glycine and taurine. These two bile acids combine with sodium and potassium to form bile salts. Bile salts are sodium or potassium taurocholate, and sodium or potassium glycocholate. Hay’s test is also called  sulphur powder test . Hay’s test for bile salts principle: Bile salts have a property of lowering the surface tension of the fluid. If Bile salts present in urine and sulphur powder is added to the urine in the test tube, the sulphur particles will sink. In normal cases it does not sink rather, it floats on the surfa

Fungal Infections

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            fungal infections Fungal infections are common throughout much of the natural world. In humans, fungal infections occur when an invading fungus takes over an area of the body and is too much for the immune system to handle. Fungi can live in the air, soil, water, and plants. There are also some fungi that live naturally in the human body. Like many microbes, there are helpful fungi and harmful fungi. When harmful fungi invade the body, they can be difficult to kill, as they can survive in the environment and re-infect the person trying to get better. In this article, we take a look at who is most at risk of getting a fungal infection and what the symptoms and treatment options are for some common types. Who is at risk? Fungal infections are common in humans and are usually not very serious if they are treated quickly and correctly. Anyone with a weakened immune system may be more likely to contract a fungal infection, as well as anyone who is taking anti

LIFE STYLE OF SCHISTOSOMA

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                                                LIFE CYCLE *the stage in the snail include two generation of SPOROCYST *after sporocyst,cercariae are produce within the snail *Upon release from the snail  the infective cercariae swim, penetret theskin of the  human host *During penetration to the human host the cercariae shed their forkedtail and become schistomalae and schistomulae migrate through several tissue and stages to their residence in the veins and mature into adult worms. *Adult worms in human reside in the mecenteric venulesin varias location , which at time seem to be specific schistosoma japonicum is more frequently found in the Superior mesenteric vein,draining the small intestine schistosoma manson occur more often the superior   mecenterih vein draining the large intestine. *Both japonicum and schistosoma manson can occupy either location and capable of moving beatween sites schistosomahaematobium most often occur in the venous plexus of bladder ,but it can als

Collection Routine Specimens

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Collection Routine Specimens Collection Routine Specimens for Laboratory Investigations   ·    SOPs (Standard Operating Procedures) o     Is the established method for performing laboratory procedures and  aim to standardise procedures in the laboratory technical work ·    Finger prick specimen o     Is a Capillary blood collected from a pierced finger ·   Venous blood sample o     Blood collected from a vein Materials Used for Collecting Venous Blood and Finger Prick   Common materials for both venous blood collection and finger prick ·           Sterile swab Used for cleaning the skin ·           70% alcohol Used for disinfecting the skin ·           Gloves Used to protect the phlebotomist from contamination ·           Disposal bucket o     Used for disposing used materials ·           Sharps box or container o     Used for disposing sharp materials Specific materials for finger prick ·           Lancet Is used for pier